Research article - Peer-reviewed, 2010
A Metabolomic Approach to Study Major Metabolite Changes during Acclimation to Limiting CO2 in Chlamydomonas reinhardtii
Renberg, Linda; Johansson, Annika I.; Shutova, Tatiana; Stenlund, Hans; Aksmann, Anna; Raven, John A.; Gardestrom, Per; Moritz, Thomas; Samuelsson, GoranAbstract
Using a gas chromatography-mass spectrometry-time of flight technique, we determined major metabolite changes during induction of the carbon-concentrating mechanism in the unicellular green alga Chlamydomonas reinhardtii. In total, 128 metabolites with significant differences between high-and low-CO2-grown cells were detected, of which 82 were wholly or partially identified, including amino acids, lipids, and carbohydrates. In a 24-h time course experiment, we show that the amino acids serine and phenylalanine increase transiently while aspartate and glutamate decrease after transfer to low CO2. The biggest differences were typically observed 3 h after transfer to low-CO2 conditions. Therefore, we made a careful metabolomic examination at the 3-h time point, comparing low-CO2 treatment to high-CO2 control. Five metabolites involved in photorespiration, 11 amino acids, and one lipid were increased, while six amino acids and, interestingly, 21 lipids were significantly lower. Our conclusion is that the metabolic pattern during early induction of the carbon-concentrating mechanism fit a model where photorespiration is increasing.Published in
Plant Physiology2010, volume: 154, number: 1, pages: 187-196
Authors' information
Renberg, Linda
Umeå University
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Shutova, Tatiana
Umeå University
Stenlund, Hans
Umeå University
Aksmann, Anna
University of Gdansk
Raven, John A.
University of Dundee
Gardeström, Per
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Samuelsson, Göran
UKÄ Subject classification
Forest Science
Publication Identifiers
DOI: https://doi.org/10.1104/pp.110.157651
URI (permanent link to this page)
https://res.slu.se/id/publ/31989