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Research article - Peer-reviewed, 2004

Serglycin is essential for maturation of mast cell secretory granule

Abrink M, Grujic M, Pejler G


To address the biological function of the scarcely studied intracellular proteoglycans, we targeted the gene for serglycin (SG), the only known committed intracellular proteoglycan. SG(-/-) mice developed normally and were fertile, but their mast cells (MCs) were severely affected. In peritoneum there was a complete absence of normal granulated MCs. Furthermore, peritoneal cells and ear tissue from SG(-/-) animals were devoid of the various MC-specific proteases. However, mRNA for the proteases was present in SG(+/+), SG(+/+), and SG(-/-) tissues, indicating that SG is essential for the storage, but not expression, of the MC proteases. Experiments, in which the differentiation of bone marrow stem cells into mature MCs was followed, showed that secretory granule maturation was compromised in SG(-/-) cells. Moreover, SG(+/+) and SG(+/-) cells, but not SG(-/-) cells, synthesized proteoglycans of high anionic charge density. Taken together, we demonstrate a key role for SG proteoglycan in MC function

Published in

Journal of Biological Chemistry
2004, volume: 279, number: 39, pages: 40897-40905

Authors' information

Swedish University of Agricultural Sciences, Department of Molecular Biosciences
Grujic, Mirjana
Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry (AFB)

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