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Conference abstract, 2010

Failure of the Advia 2120, Sysmex XT-2000iV and Cell-Dyn 3500 to detect canine basophils

Lilliehöök, Inger; Tvedten, Harold

Abstract

Currently available veterinary hematology analyzers do not detect canine basophils. A flag, numerical change or a morphologic change in instrument graphical displays is needed to suggest occult basophilia to the operator. In this study the inability of the automated basophil count of three major veterinary hematology analyzers (Advia 2120, Sysmex XT-2000iV and CELL-DYN 3500) to detect basophils was verified and the apparent location of basophils in instrument leukocyte cytograms was identified in dogs with prominent basophilia. Ten canine blood samples with prominent basophilia (≥ 5% with manual differential leukocyte count) were analyzed with one or more instruments. Sysmex XT-2000iV and Advia 2120 utilize the human basophil's resistance to lysis by a basophil reagent and subsequent larger size for detection. Canine basophils appear to lyse in these “basophil” reagents like other leukocytes and therefore Advia and Sysmex fails to detect them. Basophil counts were not increased in Sysmex XT-2000iV (9 samples), CELL-DYN 3500 (6 samples) or Advia 2120 (4 samples) in the canine samples with 5-15% of basophils with manual differential count. The probable basophil cell population was identified in the cytograms of all three instruments, therefore recognition of this pattern may suggest the presence of basophilia to the operator. Increased Advia LUC cells may also suggested basophilia. These criteria may be used to recommend a manual differential leukocyte count

Published in

Veterinary Clinical Pathology
2010, Volume: 39, number: 4, pages: 541-541

Conference

European Society for Veterinary ClinicalPathology (ESVCP) 12th Annual Congress, Toulouse, France—September 9–11, 2010