Preparation and certification of zearalenone mass concentration of two low-level maize reference materials

Abstract

The contamination of maize by fungi, especially by Fusarium species, is, a worldwide problem. One of the most prevalent Fusarium mycotoxins frequently found on European maize is zearalenone (ZON), which has been implicated in a range of human and animal diseases. It shows remarkable estrogenic properties and can cause severe infertility problems in farm animals. Currently, 9 countries have set maximum tolerable levels for ZON in food, ranging from 0 to 1000 mug/kg. This paper describes the preparation of 2 maize reference materials (BCR-716 very low level ZON and BCR-717 low level ZON) and the certification of their individual ZON contents (mass concentration and mass fraction). Uncertainties were calculated in compliance with the Guide to the Expression of Uncertainty in Measurement and include uncertainties that are due to possible inhomogeneity and instability. Finally, BCR-716 was certified at a level of <5 mug/kg and BCR-717 at a level of 83 mug/kg with an expanded uncertainty (k = 2) of 9 mug/kg

Keywords

Performance Liquid-Chromatography; Certified Reference Material; Mycotoxin Zearalenone; Fusarium Mycotoxins; Gas-Chromatography; Rice Culture; Deoxynivalenol; Stability; Spectrometry; Cleanup

Published in

Journal of AOAC INTERNATIONAL
2004, volume: 87, number: 4, pages: 892-908
Publisher: AOAC INTERNATIONAL

SLU Authors

• Pettersson, Hans

  • Department of Applied Animal Science and Welfare, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Food Science
Animal and Dairy Science
Veterinary Science

Publication identifier

• DOI: https://doi.org/10.1093/jaoac/87.4.892

Permanent link to this page (URI)

https://res.slu.se/id/publ/3323