Kylin, Henrik
- Norwegian Institute for Air Research (NILU)
Abstract
Vitamin B12, viz., cyano- or hydroxo-cobalamin, can be chemically or enzymatically converted into the derivatives methyl- and adenosyl-cobalamin, which are complex organometallic cofactors associated with several cobalamin-dependent enzymes. The reduced form of vitamin B12, cob(I)alamin {Cbl(I)}, obtained by reduction of hydroxocobalamin (OH-Cbl) with e.g. sodium borohydride, is one of the most powerful nucleophiles known. Cbl(I) was shown to react readily with the synthetic sweetener sucralose (1,6-dichloro-1,6-dideoxy-b-D-fructofuranosyl-4-chloro-4-deoxy-a-D-galactopyranoside) in an aqueous system to form an alkylcobalamin (Suc-Cbl). This occurred by replacement of one of the three chlorine atoms of sucralose with a cobalamin moiety. The efficiency of trapping sucralose in presence of excess Cbl(I) was estimated to be >90%. Furthermore, in an in vitro study using human liver S9 with NADPH regeneration, in presence of OH-Cbl and sucralose, Suc-Cbl was shown to be formed. The Suc-Cbl was characterized primarily by LC-ESI+-MS/MS. Given the human consumption of sucralose from food and beverages, such a reaction between the sweetener and reduced vitamin B12 could occur in vivo
Keywords
Vitamin B12; sucralose; NADPH; alkylcobalamin; LC-MS/MS; sukralos
Published in
Food and Chemical Toxicology
2011, volume: 49, number: 4, pages: 750-757
SLU Authors
UKÄ Subject classification
Environmental Sciences and Nature Conservation
Food Science
Publication identifier
- DOI: https://doi.org/10.1016/j.fct.2010.11.037
Permanent link to this page (URI)
https://res.slu.se/id/publ/33283