Abstract

Two surface plasmon resonance (SPR)-based biosensor assays for detection of beta-lactam antibiotics in milk are reported. The assays are based on the enzymatic activity of a carboxypeptidase converting a 3-peptide into a 2-peptide, a reaction that is inhibited in the presence of beta-lactams. Antibodies were used to measure either the amount of formed enzymatic product or the amount of remaining enzymatic substrate. Both assays detected different beta-lactams at or below European maximum residue limits (MRLs), and the detection limit for penicillin G was 1.2 mug/kg and 1.5 mug/kg for the 2- and 3-peptide assays, respectively. The precision (CV) was < 5%, both within and between assays at the penicillin G MRL (4,mug/kg). The biosensor results obtained upon analysis of incurred milk samples were compared with results obtained by liquid chromatography (HPLC), and the method agreements were, in general, good

Published in

Journal of Agricultural and Food Chemistry
2004, Volume: 52, number: 10, pages: 2791-2796
Publisher: AMER CHEMICAL SOC

SLU Authors

• Lundh, Åse

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Gustavsson, Eva

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Food Science


Publication identifier

DOI: https://doi.org/10.1021/jf0344284

Permanent link to this page (URI)

https://res.slu.se/id/publ/3650