Abstract

We have developed a protocol for rapid generation of barley plants with introduced genes by employing techniques of tissue culture, plant regeneration and crossing. Compared to conventional protocols, the time between pollination of To plants and identification of phenotypic expression in the T(1) generation is reduced from around 17-9 weeks. All of the selected candidates after the tissue culture screening exhibited phenotypic expression. Furthermore, by utilizing ordinary pollen crossing, we demonstrated a route for introduction of genes to a recalcitrant barley elite cultivar. This Tissue Culture after Crossing (TCC) procedure significantly enhances the prospects for producing transgenic barley lines from desirable germplasm. We suggest that the TCC method will contribute to improve barley as a promising bioenergy crop. (C) 2011 Elsevier Ltd. All rights reserved.

Keywords

Barley; Bioenergy; Tissue culture; Transgenics

Published in

Applied Energy
2012, Volume: 91, number: 1, pages: 405-411

SLU Authors

• Nalawade, Satish

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    
  • Mahyco Research Center

• Sun, Chuanxin

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Agricultural Science
Renewable Bioenergy Research


Publication identifier

DOI: https://doi.org/10.1016/j.apenergy.2011.09.042

Permanent link to this page (URI)

https://res.slu.se/id/publ/37552