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Book chapter2012

Applications of colloid centrifugation in Assisted Reproduction

Morrell, Jane

Abstract

Coated silica colloids have been used to prepare spermatozoa for AssistedReproduction for approximately 30 years, the colloids in question being Percoll(polyvinylpyrrolidone-coated silica) and silane-coated silica. Spermatozoa to be used inassisted reproduction technologies (ART) must be capable of normal function i.e. tofertilize and activate the oocyte and to participate in subsequent development of thezygote. Colloid centrifugation selects the sperm sub-populations that are highly motile,have normal morphology, intact membranes and good chromatin integrity from the restof the ejaculate. In most species, these parameters of sperm quality have been linked to conception rate following artificial insemination (AI). In human fertility treatment,normal morphology and chromatin integrity are related to embryo development followingin vitro fertilization (IVF). Colloid centrifugation has been used extensively to prepare spermatozoa for human assisted reproduction, where spermatozoa are selected by density gradient centrifugation (DGC) for IVF, intracytoplasmic sperm injection (ICSI) orintrauterine insemination (IUI). Since the requirements for assisted reproductiontechnologies in animals differ widely between species and treatments, different protocols have been developed to supply these needs. In animals, artificial insemination (AI) is the predominant form of assisted reproduction used, with IVF or ICSI being confined to specialists or for conservation breeding. Recent developments to take account of these species differences include a modification of DGC that uses only one layer of colloid,hence its name Single Layer Centrifugation (SLC). SLC through a species-specific colloid has been shown to be effective in selecting the best spermatozoa (spermatozoa with good motility and normal morphology) from stallion, boar and bull sperm samples.


Published in

Title: Colloids: Classification, Properties and Applications
ISBN: 978-1-62081-143-6Publisher: Nova publishers

    UKÄ Subject classification

    Animal and Dairy Science
    Veterinary Science

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/38340