Research article - Peer-reviewed, 2001
The myrosinase-glucosinolate system in the interaction between Leptosphaeria maculans and Brassica napus
Andreasson, Erik; Wretblad, Sofia; Granér, Georg; Wu, Xiaoming; Zhang, Jiaming; Dixelius, Christina; Rask, Lars; Meijer, JohanAbstract
Leptosphaeria maculans causes blackleg disease, and resistance to this fungal pathogen is an important trait in the breeding of oil-seed rape. A better comprehension of the role of the myrosinase-glucosinolate system in this context is of great value. The present study is the first to address effects on multiple components of this complex system, including concentrations of individual glucosinolates, product formation, myrosinase isoform distribution and activity, and levels of myrosinase binding proteins during the infection process. One resistant B. napus cultivar (Maluka) and one susceptible cultivar (Westar) were compared in the investigation. Our results show that the two cultivars had the same histological distribution, isoform expression, and activity of the myrosinase enzymes. The glucosinolate levels were also similar, with the exception of glucobrassicin and neoglucobrassicin, which were significantly lower in the resistant cultivar at 11 days post-infection. Growth of the fungus on the plant tissues did not alter glucosinolate levels, suggesting that L. maculans does not degrade these compounds. When the plants were starved of sulphur, and thereby depleted of glucosinolates, no increased susceptibility was observed. Hence, we suggest that the myrosinase-glucosinolate system does not determine the outcome of the interaction between B. napus and L. maculans.Published in
Molecular Plant Pathology2001, volume: 2, number: 5, pages: 281-286
Authors' information
University of Copenhagen
SLU - Swedish University of Agricultural Sciences
Wretblad, Sofia
SLU - Swedish University of Agricultural Sciences
Granér, Georg
SLU - Swedish University of Agricultural Sciences
Wu, Xiaoming
SLU - Swedish University of Agricultural Sciences
Zhang, Jiaming
SLU - Swedish University of Agricultural Sciences
SLU - Swedish University of Agricultural Sciences
Rask, Lars
SLU - Swedish University of Agricultural Sciences
Rask, Lars
Uppsala University
SLU - Swedish University of Agricultural Sciences
UKÄ Subject classification
Plant Biotechnology
Publication Identifiers
DOI: https://doi.org/10.1046/j.1464-6722.2001.00076.x
URI (permanent link to this page)
https://res.slu.se/id/publ/39073