Oskarsson, Agneta
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Abstract
A simple and rapid milking technique for mice is described by which multiple milk samples can be collected from anaesthetised dams after I h separation from the litter. Three samples of milk were collected from each dam by aspiration via a flexible tubing fitted to the nipple and approximately 0.3-0.5 ml milk was obtained at each time point. The milking technique was tested with 14 C-labelled 2,2',4,4,5-pentabromodiphenyl ether (BDE-99) as a model substance. A rapid and efficient milk excretion of BDE-99, with a milk/plasma ratio of approximately 40, was demonstrated. During the studied 7 days most of the given dose was excreted in milk with peak levels already 4-10 h after an intravenous administration. Higher tissue concentrations of BDE-99 were found in suckling neonates than in dams, probably due to a high dose per kilogram body weight from milk and an efficient absorption via the gastrointestinal tract in the pups. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
Keywords
milk collection; lactation; PBDE; polybrominated diphenyl ether
Published in
Toxicology Letters
2004, volume: 151, number: 2, pages: 327-334
Publisher: ELSEVIER SCI IRELAND LTD
SLU Authors
UKÄ Subject classification
Food Science
Publication identifier
- DOI: https://doi.org/10.1016/j.toxlet.2004.02.011
Permanent link to this page (URI)
https://res.slu.se/id/publ/3983