A method for studies on milk excretion of chemicals in mice with 2,2 ',4,4 ',5-pentabromodiphenyl ether (BDE-99) as a model

Abstract

A simple and rapid milking technique for mice is described by which multiple milk samples can be collected from anaesthetised dams after I h separation from the litter. Three samples of milk were collected from each dam by aspiration via a flexible tubing fitted to the nipple and approximately 0.3-0.5 ml milk was obtained at each time point. The milking technique was tested with 14 C-labelled 2,2',4,4,5-pentabromodiphenyl ether (BDE-99) as a model substance. A rapid and efficient milk excretion of BDE-99, with a milk/plasma ratio of approximately 40, was demonstrated. During the studied 7 days most of the given dose was excreted in milk with peak levels already 4-10 h after an intravenous administration. Higher tissue concentrations of BDE-99 were found in suckling neonates than in dams, probably due to a high dose per kilogram body weight from milk and an efficient absorption via the gastrointestinal tract in the pups. (C) 2004 Elsevier Ireland Ltd. All rights reserved

Published in

Toxicology Letters
2004, Volume: 151, number: 2, pages: 327-334 Publisher: ELSEVIER SCI IRELAND LTD

SLU Authors

• Oskarsson, Agneta

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Food Science


Publication identifier

DOI: https://doi.org/10.1016/j.toxlet.2004.02.011

Permanent link to this page (URI)

https://res.slu.se/id/publ/3983