Conference paper - Peer-reviewed, 2003
Development of a SCAR Marker Linked to Dominant Gene Conferring Resistance to Colletotrichum acutatum in Strawberry (Fragaria x ananassa)
Guerin, G; Lerceteau-Kohler, EAbstract
Colletotrichum acutatum is responsible for strawberry anthracnose, one of the main disease of cultivated strawberry in the south-western area of France (60% of the French production). Anthracnose is a polycyclic disease for which no efficient disease control method has been developed. Therefore, the development of cultivars with sustainable resistance, i.e. efficient against the two pathogenicity groups, may be promising to lower both the level of damage and the use of chemicals. The objective of this study is to identify molecular markers linked to the major dominant gene conferring resistance to pathogenicity group 2 by using an approach of Bulk Segregant Analysis (BSA) and Amplified Fragment Length Polymorphisin (AFLP) markers. We identified four molecular markers, named mf1 to mf4, linked to the resistance gene to the pathogenicity group 2 of C. acutatum. Within a segregating population (back cross type) for the dominant gene, no recombination between the resistance allele and the marker mf3 was detected in 78 BC1 individuals studied. One Sequence Characterized Amplified Region (SCAR) obtained from mf4 (SCAR-mf4) provided a fast and easy test for selection of anthracnose resistant plants at early stage, with a misidentification rate of 1.3%. The evaluation of this SCAR marker in few strawberry varieties showed that only some resistant varieties possessed this marker.Keywords
AFLPs; Anthracnose; Bulk Segregant Analysis; SCAR markerPublished in
Acta Horticulturae2003, volume: 626, number: 626, pages: 85-91
Book title: Berry crop breeding, production and utilization for new century : a proceedings of the XXVI International Horticultural Congress: Toronto, Canada, 11-17 August, 2002
ISBN: 90-6605-340-2
Publisher: International Society for Horticultural Science
Conference
XXVI International Horticultural CongressAuthors' information
Guérin, Guy
National Institute of Agricultural Research (INRA)
Lerceteau-Köhler, Estelle
Ciref
UKÄ Subject classification
Genetics
Genetics and Breeding
Publication Identifiers
DOI: https://doi.org/10.17660/ActaHortic.2003.626.10
URI (permanent link to this page)
https://res.slu.se/id/publ/40180