In Vitro Protein Degradation of 38 Sainfoin Accessions and Its Relationship to Tannin Content by Different Assays

Abstract

This study compared 38 sainfoin and 2 Lotus accessions to their respective tannin contents, N buffer solubility, and in vitro protein degradation. Tannin contents were measured by a protein precipitation method using either bovine serum albumin or Rubisco and by the colorimetric HCl/butanol method. Precipitation of bovine serum albumin and Rubisco was highly correlated (R-2 = 0.939). Correlations between the protein precipitation variants and the HCl/butanol method were relatively low (R-2 < 0.6). Protein degradation was measured at 4 h of incubation in an inhibited in vitro system and could not be explained by any of the tannin assays (R-2 < 0.03) and only partially by N buffer solubility (R-2 <= 0.433). Decisive factors other than the quantity of tannins or their ability to precipitate proteins must be considered. Resistance of soluble protein toward degradation can possibly be caused by tannin protein binding.

Keywords

Onobrychis viciifolia; Lotus corniculatus; esparsette; Rubisco; bovine serum albumin; radial diffusion assay

Published in

Journal of Agricultural and Food Chemistry
2012, volume: 60, number: 20, pages: 5071-5075
Publisher: AMER CHEMICAL SOC

SLU Authors

• Lorenz, Martin

  • Department of Applied Animal Science and Welfare, Swedish University of Agricultural Sciences
    

• Udén, Peter

  • Department of Applied Animal Science and Welfare, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Agricultural Science

Publication identifier

• DOI: https://doi.org/10.1021/jf3001179

Permanent link to this page (URI)

https://res.slu.se/id/publ/40437