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Research article - Peer-reviewed, 2011

The Putative Endoglucanase PcGH61D from Phanerochaete chrysosporium Is a Metal-Dependent Oxidative Enzyme that Cleaves Cellulose

Sandgren, Mats; Wu, Miao; Ishida, Takuya; Ståhlberg, Jerry

Abstract

Many fungi growing on plant biomass produce proteins currently classified as glycoside hydrolase family 61 (GH61), some of which are known to act synergistically with cellulases. In this study we show that PcGH61D, the gene product of an open reading frame in the genome of Phanerochaete chrysosporium, is an enzyme that cleaves cellulose using a metal-dependent oxidative mechanism that leads to generation of aldonic acids. The activity of this enzyme and its beneficial effect on the efficiency of classical cellulases are stimulated by the presence of electron donors. Experiments with reduced cellulose confirmed the oxidative nature of the reaction catalyzed by PcGH61D and indicated that the enzyme may be capable of penetrating into the substrate. Considering the abundance of GH61-encoding genes in fungi and genes encoding their functional bacterial homologues currently classified as carbohydrate binding modules family 33 (CBM33), this enzyme activity is likely to turn out as a major determinant of microbial biomass-degrading efficiency.

Published in

PLoS ONE
2011, volume: 6, number: 11
Publisher: PUBLIC LIBRARY SCIENCE

Authors' information

Swedish University of Agricultural Sciences, Department of Molecular Biology
Wu, Miao
Swedish University of Agricultural Sciences, Department of Molecular Biology
Ishida, Takuya
Swedish University of Agricultural Sciences, Department of Molecular Biology

UKÄ Subject classification

Biochemistry and Molecular Biology

Publication Identifiers

DOI: https://doi.org/10.1371/journal.pone.0027807

URI (permanent link to this page)

https://res.slu.se/id/publ/40783