Separation of viable and non-viable filled Scots pine seeds by differentiating between drying rates using single seed near infrared transmittance spectroscopy

Abstract

Single seed near infrared (NIR) transmittance spectroscopy showed high potential to classify and separate viable seeds from non-viable filled seeds of Scots pine (Pinus sylvestris L.). Multivariate calibration models based on NIR transmittance spectroscopy in the range 850-1048 nm had high prediction accuracy (98-99%) for the characterisation and separation of viable and non-viable filled Scots pine seeds. Viable and non-viable filled seeds were imbibed to 30% moisture content for 4 days at 15degreesC and nearly 100% relative air humidity. Utilising the phenomenon of different drying rates of imbibed viable and non-viable filled seeds, calibration models were fitted based on single seed spectra collected after 0.8-3.8 h of drying time at ca 20degreesC and ca 25% relative air humidity. The high prediction accuracy of classifying viable and non-viable filled seeds in the test set, dried for 2 h, was achieved using PLS regression modelling of NIR spectra collected within the calibration set.

Keywords

Softwood forest tree; Reflection spectrometry; Spermatophyta; Gymnospermae; Coniferales; Water content; Pinus sylvestris; Multivariate analysis; Calibration; Forecast model; Near infrared spectrometry; Drying; Imbibition; Separation process; Viability; Seed

Published in

Seed Science and Technology
2002, volume: 30, number: 2, pages: 383-392
Publisher: ISTA

SLU Authors

• Lestander, Torbjörn

  • Department of Silviculture, Swedish University of Agricultural Sciences
    

• Oden, Per

  • Department of Silviculture, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Forest Science

Permanent link to this page (URI)

https://res.slu.se/id/publ/40869