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Research article - Peer-reviewed, 2002

Separation of viable and non-viable filled Scots pine seeds by differentiating between drying rates using single seed near infrared transmittance spectroscopy

Lestander, Torbjörn; Oden, Per Christer


Single seed near infrared (NIR) transmittance spectroscopy showed high potential to classify and separate viable seeds from non-viable filled seeds of Scots pine (Pinus sylvestris L.). Multivariate calibration models based on NIR transmittance spectroscopy in the range 850-1048 nm had high prediction accuracy (98-99%) for the characterisation and separation of viable and non-viable filled Scots pine seeds. Viable and non-viable filled seeds were imbibed to 30% moisture content for 4 days at 15degreesC and nearly 100% relative air humidity. Utilising the phenomenon of different drying rates of imbibed viable and non-viable filled seeds, calibration models were fitted based on single seed spectra collected after 0.8-3.8 h of drying time at ca 20degreesC and ca 25% relative air humidity. The high prediction accuracy of classifying viable and non-viable filled seeds in the test set, dried for 2 h, was achieved using PLS regression modelling of NIR spectra collected within the calibration set.


Softwood forest tree; Reflection spectrometry; Spermatophyta; Gymnospermae; Coniferales; Water content; Pinus sylvestris; Multivariate analysis; Calibration; Forecast model; Near infrared spectrometry; Drying; Imbibition; Separation process; Viability; Seed

Published in

Seed Science and Technology
2002, Volume: 30, number: 2, pages: 383-392
Publisher: ISTA

      SLU Authors

      • Oden, Per

        • Department of Silviculture, Swedish University of Agricultural Sciences

      UKÄ Subject classification

      Forest Science

      Permanent link to this page (URI)