Research article - Peer-reviewed, 2011
Changes in arterial PO2, physiological blood parameters and intracellular antioxidants in free-swimming Atlantic cod (Gadus morhua) exposed to varying levels of hyperoxia
Karlsson Anders , Heier, Lene Sørlie; Rosseland, Bjørn Olav; Salbu, Brit; Kiessling, Anders
Abstract
Free-swimming Atlantic cod (Gadus morhua) were exposed to water oxygen pressures (P O-w(2)) ranging from 18.1 to 41.5 kPa and sampled for blood using an indwelling caudal artery cannula. Arterial blood oxygen pressure (P O-a(2)) increased with increasing P O-w(2), from 12.0 kPa in normoxia (18.1 kPa) to 34.2 kPa in the highest hyperoxic level tested (41.5 kPa). Blood CO2 pressure and plasma bicarbonate concentration increased with P O-w(2), indicating reduced ventilation with increased P O-w(2). Plasma glucose, sodium and potassium were not affected by water oxygen level. Blood oxidative stress biomarkers, reduced glutathione, oxidized glutathione and the oxidative stress index (ratio between oxidized and total glutathione) differed intermittently between normoxia and hyperoxia. The oxidative stress index was higher in the blood of exposed compared to unexposed control cod. Together with elevated P O-a(2), these findings suggest increased production of reactive oxygen species and increased oxidative stress in Atlantic cod exposed to hyperoxia.
Keywords
Oxygen supersaturation; Normoxia; Cannulation; Oxidative stress; Glutathione; Oxidation
Published in
Fish Physiology and Biochemistry
2011, Volume: 37, number: 1, pages: 249-258
Publisher: Springer Verlag (Germany)
SLU Authors
UKÄ Subject classification
Fish and Aquacultural Science
Publication Identifiers
DOI: https://doi.org/10.1007/s10695-010-9438-z
Permanent link to this page (URI)
https://res.slu.se/id/publ/41060