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Research article2012Peer reviewedOpen access

Exit from Proliferation during Leaf Development in Arabidopsis thaliana: A Not-So-Gradual Process

Andriankaja, Megan; Dhondt, Stijn; De Bodt, Stefanie; Vanhaeren, Hannes; Coppens, Frederik; De Milde, Liesbeth; Muehlenbock, Per; Skirycz, Aleksandra; Gonzalez, Nathalie; Beemster, Gerrit T. S.; Inze, Dirk

Abstract

Early leaf growth is sustained by cell proliferation and subsequent cell expansion that initiates at the leaf tip and proceeds in a basipetal direction. Using detailed kinematic and gene expression studies to map these stages during early development of the third leaf of Arabidopsis thaliana, we showed that the cell-cycle arrest front did not progress gradually down the leaf, but rather was established and abolished abruptly. Interestingly, leaf greening and stomatal patterning followed a similar basipetal pattern, but proliferative pavement cell and formative meristemoid divisions were uncoordinated in respect to onset and persistence. Genes differentially expressed during the transition from cell proliferation to expansion were enriched in genes involved in cell cycle, photosynthesis, and chloroplast retrograde signaling. Proliferating primordia treated with norflurazon, a chemical inhibitor of retrograde signaling, showed inhibited onset of cell expansion. Hence, differentiation of the photosynthetic machinery is important for regulating the exit from proliferation.

Published in

Developmental Cell
2012, Volume: 22, number: 1, pages: 64-78 Publisher: CELL PRESS

UKÄ Subject classification

Botany

Publication identifier

DOI: https://doi.org/10.1016/j.devcel.2011.11.011

Permanent link to this page (URI)

https://res.slu.se/id/publ/41173