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Research article - Peer-reviewed, 2001

Conserved interaction between distinct Kruppel-associated box domains and the transcriptional intermediary factor 1 beta

Åbrink, Magnus; Ortiz, José A.; Mark, Charlotta; Sanchez, Cecilia; Looman, Camilla; Hellman, Lars; Chambon, Pierre; Losson, Régine


The Kruppel-associated box (KRAB) domain, originally identified as a 75-aa sequence present in numerous Kruppel-type zinc-finger proteins, is a potent DNA-binding-dependent transcriptional repression domain that is believed to function through interaction with the transcriptional intermediary factor 1 (TIF1) beta. On the basis of sequence comparison and phylogenetic analysis, we have recently defined three distinct subfamilies of KRAB domains. In the present study, individual members of each subfamily were tested for transcriptional repression and interaction with TIF1 beta and two other closely related family members (TIF1 alpha and TIF1 gamma). All KRAB variants were shown, (i) to repress transcription when targeted to DNA through fusion to a heterologous DNA-binding domain in mammalian cells, and (ii) to interact specifically with TIF1 beta, but not with TIF1 alpha Or TIF1 gamma. Taken together, these results implicate TIF1 beta as a common transcriptional corepressor for the three distinct subfamilies of KRAB zinc-finger proteins and suggest a high degree of conservation in the molecular mechanism underlying their transcriptional repression activity.

Published in

Proceedings of the National Academy of Sciences
2001, volume: 98, number: 4, pages: 1422-1426

Authors' information

Uppsala University
Ortiz, José A.
University Miguel Hernandez of Elche (UMH)
Mark, Charlotta
Uppsala University
Sanchez, Cecilia
The National Center for Scientific Research (CNRS)
Looman, Camilla
Uppsala University
Hellman, Lars
Uppsala University
Chambon, Pierre
The National Center for Scientific Research (CNRS)
Losson, Régine
The National Center for Scientific Research (CNRS)

UKÄ Subject classification

Cell and Molecular Biology

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