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Research article - Peer-reviewed, 1995

Isolation of cDNA Clones for 42 Different Krüppel-Related Zinc Finger Proteins Expressed in the Human Monoblast Cell Line U-937

Åbrink, Magnus; Aveskogh, M.; Hellman, Lars


To study the complexity and structural characteristics of zinc finger proteins expressed during human hematopoiesis and to isolate novel regulators of blood cell development, a degenerate oligonucleotide probe specific for a consensus zinc finger peptide domain was used to isolate 63 cDNA clones for Kruppel-related zinc finger genes from the human monoblast cell line U-937. By extensive nucleotide sequence and Northern blot analysis, these cDNA clones were found to originate from approximately 42 different genes (HZF 1-42) of which only 8 have previously been described. Northern blot analysis showed that a majority of these genes were expressed at comparable levels in U-937 and HeLa cells. The large number of individual genes represented among the 63 clones and their apparent non-cell-type-specific expression suggest that the majority of the Kruppel-related zinc finger genes are likely to be expressed in most human tissues. In contrast, some of the genes displayed a restricted expression pattern, indicating that the represent potential regulators of monocyte differentiation or proliferation. Detailed structural analysis of the first 12 cDNAs (HZF 1-10) and a partial characterization of HZF 11-42 revealed that a common feature of human Kruppel-related zinc finger proteins is the presence of tandem arrays of zinc fingers ranging in number from 3 to over 20 that are preferentially located in the carboxy-terminal regions of the proteins. In addition, several novel KRAB-containing zinc finger genes and a novel conserved sequence element were identified.

Published in

DNA and Cell Biology
1995, volume: 14, number: 2, pages: 125-136

Authors' information

Uppsala University
Aveskogh, M.
Uppsala University
Hellman, Lars
Uppsala University

UKÄ Subject classification

Biochemistry and Molecular Biology

Publication Identifiers


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