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Research article - Peer-reviewed, 2013

Enumeration of feline platelets in ethylenediamine tetra-acetic acid anti-coagulated blood with the ADVIA 2120 and two manual methods: Leucoplate and Thrombo-TIC

Tvedten, Harold; Ljusner, Jessika; Lilliehöök, Inger

Abstract

A manual method (Thrombo-TIC) was advertized by the company to disaggregate platelet clumps and to make human platelets spherical to improve platelet enumeration. The study's hypothesis was that this method would perform better than current methods for feline blood anti-coagulated with EDTA which often contains platelet aggregates. Platelet concentrations were determined in 21 feline blood samples by 3 methods. Thrombo-TIC was compared to the laboratory's current manual method (Leucoplate) and an Advia 2120 optical platelet concentration. Feline blood samples often contained platelet aggregates. Advia flagged for platelet aggregates in 11 of the 21 feline blood samples and examination of blood smear revealed platelet aggregates in 14 of the 21 samples. The hemocytometers had more platelet aggregates seen with the Thrombo-TIC method than with Leucoplate. The method giving the greatest platelet concentration was considered most accurate. Leucoplate's median platelet concentration (238 x 10⁹/L) was greater than Thrombo-TIC (202 x 10⁹/L) or Advia (157 x 10⁹/L). Intra-assay precision was determined for the 3 methods for the 21 feline blood samples. Median Thrombo-TIC and Leucoplate precision (7.4 and 7.3 % CV) were similar and not much worse than Advia's (5.9 % CV). Thrombo-TIC method did not appear to perform better than the current manual method (Leucoplate). Leucoplate appeared least affected by platelet aggregation in feline blood. Advia's automated platelet concentration appeared to be most negatively affected by platelet aggregation. [A1]Thrombo-TIC method did not appear to prevent platelet aggregation in feline EDTA blood samples and is not recommended for such use. [A1]You end this abstract with a slew of results. Normally, an abstract should end with a statement on the implication or significance of your results.

Keywords

Cats; platelet aggregate; platelet concentration

Published in

Journal of Veterinary Diagnostic Investigation
2013, Volume: 25, number: 4, pages: 493-497