Madawala, Samanthi
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Research article2012Peer reviewedOpen access
Madawala, Samanthi; Andersson, Rolf; Jastrebova, Jelena; Almeida, Maria; Dutta, Paresh; Madawala, Samanthi
Plant sterols (PS) are bioactive compounds effective in reducing plasma cholesterol. Fatty acid esters of PS have improved solubility and blending properties when utilized in various food products. Naturally occurring a-lipoic acid (LA) and its reduced form dihydrolipoic acid (DHLA) are known for their antioxidant activity. In addition, they have shown an array of health beneficial properties against obesity, diabetes, cancer, cardiovascular and inflammatory diseases etc. Different LA conjugates have been reported to have increased bioactivity compared to the parent compounds. The objective of this study was to synthesize PS esters of LA (PSLA) and DHLA (PSDHLA) in order to increase their cholesterol lowering effect and reducing the risk of atherosclerosis with additional health benefits e.g. against oxidative stress.Synthesis of PSLA and PSDHLA was performed with a pure PS mixture of beta-sitosterol, stigmasterol, campesterol and brassicasterol. The free radical scavenging capacity of the conjugates was assessed by the DPPH method. Remaining percentage of DPPH free radicals was measured at the steady state for different concentrations of PSLA and PSDHLA. High free radical scavenging capacity was observed for PSDHLA compared with PSLA. Efficient concentration EC50 as a molar ratio for PSDHLA was 0.43. The derivatives were analyzed by RP-HPLC-MS-APCI. The order of the elution times of the compounds observed in HPLC-MS analysis was PS< PSDHLA< PSLA. Baseline separation was not achieved between campesterol and stigmasterol and their derivatives. These compounds could be identified by their characteristic fragment ions from the mass spectral data.
Polish Journal of Food and Nutrition Sciences
2012, Volume: 62, number: 3, pages: 159-169
SDG3 Ensure healthy lives and promote well-being for all at all ages
Analytical Chemistry
Food Science
Organic Chemistry
DOI: https://doi.org/10.2478/v10222-011-0050-4
https://res.slu.se/id/publ/41973