Expression Profiling of Muscle Reveals Transcripts Differentially Expressed in Muscle That Affect Water-Holding Capacity of Pork

Abstract

To identify biological processes as well as molecular markers for drip loss, a parameter for water holding capacity of meat, the M. longissimus dorsi transcriptomes of six divergent sib pairs were analyzed using Affymetrix Porcine Genome Array. Functional categories of differentially regulated transcripts were determined by single-gene analysis and gene set analysis. The transcripts being up-regulated at high drip loss belong to groups of genes functionally categorized as genes of membrane proteins, signal transduction, cell communication, response to stimulus, and cytoskeleton. Among genes down-regulated with high drip loss, functional groups of oxidoreductase activity, lipid metabolism, and electron transport were identified. Differential regulation of the abundance of transcripts of these biological networks in live muscle affect mortem biochemical processes of meat maturation. Knowledge of this functional link is indicative for the identification of candidate genes for improvement of meat quality.

Keywords

Gene expression; microarray; gene set analysis; muscle; pig; drip loss

Published in

Journal of Agricultural and Food Chemistry
2008, volume: 56, number: 21, pages: 10311-10317
Publisher: AMER CHEMICAL SOC

SLU Authors

• Jonas, Elisabeth

  • University of Bonn

UKÄ Subject classification

Animal and Dairy Science

Publication identifier

• DOI: https://doi.org/10.1021/jf800881y

Permanent link to this page (URI)

https://res.slu.se/id/publ/42073