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Research article2001Peer reviewed

Overexpression of the endogenous peroxidase-like gene spi 2 in transgenic Norway spruce plants results in increased total peroxidase activity and reduced growth

Elfstrand, Malin; Fossdal, Carl Gunnar; Sitbon, Folke; Olsson, O.; Lönneborg, Anders; Von Arnold, Sara


Peroxidases constitute a large family of proteins found in all higher plants. Owing to the complexity of the peroxidase isoenzyme family it has been difficult to assess the precise function of individual peroxidase enzymes. In this work we have studied the effects of an endogenous peroxidase-like gene from Norway spruce [Picea abies (L.) Karst], spi2, on the development and growth of Norway spruce somatic embryo plants. Embryogenic cells of Norway spruce transformed with spi2 under control of the maize ubi-1 promoter showed up to 40 times higher total peroxidase activity than the control cells; regenerated plants overexpressing spi2 showed an increased total peroxidase activity. Based on these results and the overall sequence similarity with cationic peroxidases we conclude that spi2 encodes a peroxidase. Overexpression of spi2 resulted in increased sensitivity to stress, leading to a reduction in epicotyl formation and in height growth compared with control plants. The plants overexpressing spi2 also showed a deeper phloroglucinol staining but similar levels of Klason lignin.


peroxidase; Norway spruce; transformation

Published in

Plant Cell Reports
2001, Volume: 20, number: 7, pages: 596-603

      SLU Authors

    • Elfstrand, Malin

      • Department of Forest Genetics, Swedish University of Agricultural Sciences
        • Von Arnold, Sara

          • Department of Forest Genetics, Swedish University of Agricultural Sciences

        UKÄ Subject classification

        Plant Biotechnology
        Other Biological Topics

        Publication identifier


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