Abstract

A microscale technique has been developed for routine quantifications of picogram amounts of indole-3-acetic acid (IAA) in plant tissues by combined gas chromatography-mass spectrometry. Low-and high-resolution selected-ion-monitoring and selected-reaction-monitoring mass spectrometry techniques were compared for selectivity and precision. The best selectivity was obtained with selected-reaction-monitoring analysis, and 1-mg samples containing 500 fg of IAA could be analyzed accurately with this method. This technique was used to investigate the IAA distribution pattern along the longitudinal axis of tobacco (Nicotiana tabacum [L.]) leaves. In young, developing leaves an increase of endogenous IAA from the leaf tip to the base of the leaf was observed, whereas the level of IAA was uniform along this axis in mature leaves.

Published in

Plant Physiology
1995, Volume: 108, number: 3, pages: 1043-1047
Publisher: AMER SOC PLANT PHYSIOLOGISTS

SLU Authors

• Sundberg, Björn

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

• Moritz, Thomas

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

• Sandberg, Göran

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Botany


Publication identifier

DOI: https://doi.org/10.1104/pp.108.3.1043

Permanent link to this page (URI)

https://res.slu.se/id/publ/42899