Abstract

Qualitative gas chromatography-mass spectrometry (GC-MS) analysis of purified extracts from 4-d-germinated seeds of ''jacaranda do cerrodo'' (Dalbergia dolichopetala) detected gibberellin A(1) (GA(1)), epi-GA(1), GA(3), GA(4), GA(5), GA(8), GA(20) and GA(28). Quantitative analysis, using deuterated internal standards, showed that the germinated seed contained 29 ng GA(1), 0.6 ng GA(4), 0.08 ng GA(5) and 1.7 ng GA(20) . g(-1) fresh weight. In metabolism experiments with germinated D. dolichopetala seeds, using H-2(2), H-3-labelled GA(1), GA(4), GA(5) and GA(20), metabolic profiles were obtained by reversed-phase high-performance liquid chromatography-radiocounting after which individual radiolabelled peaks were analysed by GC-MS and/or combined high-performance liquid chromatography-frit fast atom bombardment-mass spectrometry. The data obtained demonstrated the conversion of GA(20) to GA(20) glucosyl ester, and also to GA(1) which was further metabolised, presumably via GA(8), to an unidentified GA(8)-O-glucoside with a mass spectrum different from those of the 2-O- and 13-O-glucosides of GA(8). Gibberellin A(1) was also conjugated to form GA(1)-3 beta-O-glucoside and a conjugate identified tentatively as GA(1)-3 beta-O-glucuronic methyl ester. Gibberellin A(4) was converted to GA(4)-3 beta-O-glucoside and GA(1). Gibberellin A(5) was converted to GA(5) glucosyl ester, GA(3) and GA(3)-3 beta-O-glucoside.

Keywords

GIBBERELLIN (METABOLISM, CONJUGATION); DALBERGIA; SEED, GERMINATING

Published in

Planta
1994, Volume: 193, number: 1, pages: 1-8
Publisher: SPRINGER VERLAG

SLU Authors

• Moritz, Thomas

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Botany


Publication identifier

DOI: https://doi.org/10.1007/BF00191599

Permanent link to this page (URI)

https://res.slu.se/id/publ/42902