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Research article1992Peer reviewed

Detection and identification of gibberellins in Douglas fir (Pseudotsuga menziesii ) shoots

Doumas, P.; Moritz, Thomas; Imbault, N.; Oden, Per Christer

Abstract

Extracts of Douglas fir (Pseudotsuga menziesii [Mirb.] Franco) shoots were purified by reversed and normal phase HPLC; gibberellin (GA)-like compounds detected by radioimmunoassay with antibodies against GA4 and the Tan-ginbozu dwarf rice micro-drop bioassay were analyzed by GC-MS. Three major components were identified as GA4, GA7 and GA9 while smaller amounts of GA1, GA3 and putative GA9-glucosyl ester were also present.

Keywords

DOUGLAS FIR; GIBBERELLINS; GC-MS; IDENTIFICATION; PSEUDOTSUGA-MENZIESII

Published in

Physiologia Plantarum
1992, Volume: 85, number: 3, pages: 489-494
Publisher: MUNKSGAARD INT PUBL LTD