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Research article - Peer-reviewed, 1993

Separation and identification of cytokinins using combined capillary liquid chromatography/mass spectrometry

Imbault, Nadine; Moritz, Thomas; Nilsson, Ove; Chen, Hao‐Jie; Bollmark, Marie; Sandberg, Göran

Abstract

Non-derivatized cytokinins were analysed by liquid chromatography/mass spectrometry (LC/MS). The effluent from a capillary reversed-phase high-performance liquid chromatography column was introduced into the ion source of a double-focusing mass spectrometer via a frit interface, and ions were generated by fast atom bombardment (FAB) with 1% glycerol in the mobile phase acting as a matrix. Positive FAB spectra were obtained for base, riboside, ribotide and glucoside forms of cytokinins. The spectra were found to provide useful information for identification and structural elucidation of cytokinins. The LC/MS system was used to identify iso-pentenyladenosine in a purified extract from Norway spruce (Picea abies) needles. Quantitative analysis of iso-pentenyladenosine using (H-2(6))isopentenyladenosine as internal standard indicated levels of 1.2 ng g-1 fresh weight.

Published in

Biological Mass Spectrometry
1993, volume: 22, number: 3, pages: 201-210
Publisher: JOHN WILEY & SONS LTD

Authors' information

Imbault, Nadine
SLU - Swedish University of Agricultural Sciences
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Chen, Hao‐Jie
Stockholm University
Bollmark, Marie
Stockholm University
Sandberg, Göran
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology

UKÄ Subject classification

Analytical Chemistry

Publication Identifiers

DOI: https://doi.org/10.1002/bms.1200220310

URI (permanent link to this page)

https://res.slu.se/id/publ/42945