The Use of Combined Capillary Liquid Chromatography/Mass Spectrometry for the Identification bf a Gibberellin Glucosyl Conjugate

Abstract

Gibberellin A9 (GA9) glucosyl ester was identified in shoots of Sitka spruce [Picea sitchensis (Bong.) Carr.] grafts using capillary reversed phase high performance liquid chromatography combined on-line with a double focusing mass spectrometer. The column effluent was introduced into the ion source of the mass spectrometer via a frit-fast atom bombardment liquid chromatography/mass spectrometry interface. Negative ion mass spectra were obtained and compared with the spectrum of an authentic standard. In order to confirm the identity of the aglycone, a daughter ion spectrum from the parent ion m/z 315 was obtained by B/E linked scanning and compared with a daughter ion spectrum of free GA9. Furthermore, the elemental composition of the GA conjugate was determined by high resolution mass spectrometry.

Keywords

LIQUID CHROMATOGRAPHY MASS SPECTROMETRY; FAST ATOM BOMBARDMENT; GIBBERELLIN CONJUGATE; PICEA-SITCHENSIS, SITKA SPRUCE

Published in

Phytochemical Analysis
1992, volume: 3, number: 1, pages: 32-37
Publisher: JOHN WILEY & SONS LTD

SLU Authors

• Moritz, Thomas

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Analytical Chemistry

Publication identifier

• DOI: https://doi.org/10.1002/pca.2800030106

Permanent link to this page (URI)

https://res.slu.se/id/publ/42948