Moritz, Thomas
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
Research article1992Peer reviewed
Moritz, Thomas
Gibberellin A9 (GA9) glucosyl ester was identified in shoots of Sitka spruce [Picea sitchensis (Bong.) Carr.] grafts using capillary reversed phase high performance liquid chromatography combined on-line with a double focusing mass spectrometer. The column effluent was introduced into the ion source of the mass spectrometer via a frit-fast atom bombardment liquid chromatography/mass spectrometry interface. Negative ion mass spectra were obtained and compared with the spectrum of an authentic standard. In order to confirm the identity of the aglycone, a daughter ion spectrum from the parent ion m/z 315 was obtained by B/E linked scanning and compared with a daughter ion spectrum of free GA9. Furthermore, the elemental composition of the GA conjugate was determined by high resolution mass spectrometry.
LIQUID CHROMATOGRAPHY MASS SPECTROMETRY; FAST ATOM BOMBARDMENT; GIBBERELLIN CONJUGATE; PICEA-SITCHENSIS, SITKA SPRUCE
Phytochemical Analysis
1992, Volume: 3, number: 1, pages: 32-37
Publisher: JOHN WILEY & SONS LTD
Analytical Chemistry
DOI: https://doi.org/10.1002/pca.2800030106
https://res.slu.se/id/publ/42948