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Research article - Peer-reviewed, 2002

Altered lignin structure and resistance to pathogens in spi 2-expressing tobacco plants

Elfstrand, M; Sitbon, F; Lapierre, C; Bottin, A; von Arnold, S


The physiological role of the Norway spruce [Picea abies (L.) Karst.] spi 2 gene, encoding a defense-related cationic peroxidase was examined in transgenic tobacco (Nicotiana tabacum L.). Expression of spi 2, under control of the 35S promoter., in tobacco plants resulted in higher total peroxidase activities. The phenotype of the spi 2-transformed lines was normal. The spi 2-transformed lines displayed lignin levels similar to levels in the control line, but with some alteration in lignin histochemistry and structure. These changes were associated with reduced flexibility of the tobacco stems. The defense against pathogenic microorganisms was altered in the transgenic tobacco plants compared with control plants. High peroxidase activities increased the susceptibility to the pathogenic oomycete Phytophthora parasitica var. nicotianae, but increased the ability of the tobacco plants to suppress growth of the pathogenic bacterium Enwinia carotovora.


coniferaldehyde; defense; Erwinia; lignin; Nicotiana; peroxidase; Phytophtora

Published in

2002, volume: 214, number: 5, pages: 708-716

Authors' information

Swedish University of Agricultural Sciences, Department of Forest Genetics
Swedish University of Agricultural Sciences, Department of Plant Biology
Lapierre, Catherine
National Institute of Agricultural Research (INRA)
Bottin, Arnaud
Paul Sabatier University, Toulouse III
Swedish University of Agricultural Sciences, Department of Forest Genetics

UKÄ Subject classification

Genetics and Breeding
Biochemistry and Molecular Biology
Agricultural Science

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