Abstract

Proteomic effect screening in zebrafish liver was performed to generate hypotheses following exposure (21 days) to a structurally diverse mixture of brominated flame retardants (BFRs). Fish were exposed to two doses (10 and 100 nmol/g feed). Two-dimensional gel-electrophoresis, image analysis and MALDI-TOF mass-spectrometry revealed 13 and 19 significant responses in males and females, respectively. Effects on proteins related to cellular maintenance and stress were observed in both genders. Regulated proteins were gender-specific, but functionally indicated common protective responses (peroxiredoxin 6 and Zgc:92891 in males and transketolase in females) suggesting oxidative stress. Betaine homocysteine methyltransferase (BHMT) was induced in both genders. In addition a female-specific downregulation of iron homeostatic proteins (iron-regulatory protein 1 and transferfin) were observed. Our proteomic approach revealed novel responses that suggest important gender-specific sensitiviy to BFRs that should be considered when interpreting adverse effects of BFRs. (c) 2007 Elsevier Inc. All rights reserved.

Keywords

fish; proteomics; BFR; MALDI-TOF

Published in

Ecotoxicology and Environmental Safety
2008, Volume: 71, number: 2, pages: 319-327
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE

SLU Authors

• Norman Haldén, Anna

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Norrgren, Leif

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

Sustainable Development Goals

SDG14 Conserve and sustainably use the oceans, seas and marine resources for sustainable development


UKÄ Subject classification

Pathobiology


Publication identifier

DOI: https://doi.org/10.1016/j.ecoenv.2007.12.010

Permanent link to this page (URI)

https://res.slu.se/id/publ/43006