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Research article - Peer-reviewed, 2012

Evaluation of cytologic findings in feline conjunctivitis

Hillström, Anna; Tvedten, Harold; Källberg, Maria; Hanas, Sofia; Lindhe, Anna; Ström Holst, Bodil


Background Cytologic examination of smears prepared from ocular swabs of conjunctiva from cats with conjunctivitis permits identification of the type of inflammation and possibly specific microorganisms. Results of studies of the diagnostic utility of cytology for detection of infectious causes of feline conjunctivitis have been inconsistent. Objectives The objectives of this study were to describe cytologic findings in cats with conjunctivitis and to compare those findings with results of PCR analysis for feline herpesvirus (FHV-1), Chlamydophila felis (C felis), and Mycoplasma felis (M felis). Methods Conjunctival smears from 88 cats with conjunctivitis and 10 healthy control cats were stained with a Romanowsky stain and evaluated for the type of inflammation and evidence of an infectious agent. PCR analysis for FHV-1, C felis, and M felis was performed. Results Infectious agents identified by PCR analysis were FHV-1 in 9 cats (10%), C felis in 8 cats (9%), and M felis in 6 cats (7%). Inclusions interpreted as chlamydial inclusions were found in all cytologic smears from cats positive for C felis by PCR analysis and in 3 PCR-negative cats. Inclusions interpreted as Mycoplasma organisms were found in 3 of 6 cats that were PCR-positive for M felis and in 1 PCR-negative cat. FHV-1 inclusion bodies were not detected on cytologic examination. Conclusions Cytologic examination can be diagnostic for C felis infection when many typical inclusions are present. Cytologic examination was unreliable in diagnosing M felis infection, and viral inclusions of FHV-1 were not found in specimens stained with Romanowsky stains.


Cat; Chlamydophila felis; cytology; feline herpesvirus; Mycoplasma felis

Published in

Veterinary Clinical Pathology
2012, volume: 41, number: 2, pages: 283-290

Authors' information

Swedish University of Agricultural Sciences, Department of Clinical Sciences
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Källberg, Maria
Hanas, Sofia
Lindhe, Anna
Swedish University of Agricultural Sciences, Department of Clinical Sciences

UKÄ Subject classification

Veterinary Science
Animal and Dairy Science
Clinical Science

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