Abstract

Meat extracts with acid-soluble glycogen (macroglycogen) from M. longissmus dorsi of carriers and noncarriers of the PRKAG3 mutation (RN- and rn(+) genotype) were analyzed by both H-1 liquid-state NMR spectroscopy and a biochemical method. The H-1 NMR analysis revealed that shorter polymers (dimers, trimers, etc.) of alpha-1,4-linked glucose were generated 24-48 h post-mortem. This is not possible to elucidate with the biochemical method, by which only the total amount of hydrolyzed glucose residues is determined. The shorter polymers were primarily formed in carriers of the PRKAG3 mutation, suggesting different postmortem glycogen degradation mechanisms in the two genotypes.

Keywords

glycogen; H-1 nuclear magnetic resonance (H-1 NMR); enzymatic analysis; pork; RN-gene; PRKAG3 mutation

Published in

Journal of Agricultural and Food Chemistry
2011, Volume: 59, number: 22, pages: 11895-11902
Publisher: American Chemical Society

SLU Authors

• Essen-Gustavsson, Birgitta

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Jensen Waern, Marianne

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Lindahl, Gunilla

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Food Science
Animal and Dairy Science
Clinical Science


Publication identifier

DOI: https://doi.org/10.1021/jf201822p

Permanent link to this page (URI)

https://res.slu.se/id/publ/43199