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Research article - Peer-reviewed, 2012

Metabolomics reveals the metabolic shifts following an intervention with rye bread in postmenopausal women - a randomized control trial

Moazzami, Ali; Bondia-Pons, Isabel; Hanhineva, Kati; Juntunen, Katri; Antl, Nadja; Poutanen, Kaisa; Mykkänen, Hannu


BackgroundEpidemiological studies have consistently shown that whole grain (WG) cereals can protect against the development of chronic diseases, but the underlying mechanism is not fully understood. Among WG products, WG rye is considered even more potent because of its unique discrepancy in postprandial insulin and glucose responses known as the rye factor. In this study, an NMR-based metabolomics approach was applied to study the metabolic effects of WG rye as a tool to determine the beneficial effects of WG rye on human health. MethodsThirty-three postmenopausal Finnish women with elevated serum total cholesterol (5.0-8.5 mmol/L) and BMI of 20-33 kg/m2 consumed a minimum of 20% of their daily energy intake as high fiber WG rye bread (RB) or refined wheat bread (WB) in a randomized, controlled, crossover design with two 8-wk intervention periods separated by an 8-wk washout period. At the end of each intervention period, fasting serum was collected for NMR-based metabolomics and the analysis of cholesterol fractions. Multilevel partial least squares discriminant analysis was used for paired comparisons of multivariate data. ResultsThe metabolomics analysis of serum showed lower leucine and isoleucine and higher betaine and N,N-dimethylglycine levels after RB than WB intake. To further investigate the metabolic effects of RB, the serum cholesterol fractions were measured. Total- and LDL-cholesterol levels were higher after RB intake than after WB (p<0.05). ConclusionsThis study revealed favorable shifts in branched amino acid and single carbon metabolism and an unfavorable shift in serum cholesterol levels after RB intake in postmenopausal women, which should be considered for evaluating health beneficial effects of rye products.


Whole grain rye; Wheat; Metabolomics; NMR; Amino acid; Homocysteine; Postmenopausal; Single carbon metabolism

Published in

Nutrition Journal
2012, volume: 11, number: 88, pages: 1-8

Authors' information

Swedish University of Agricultural Sciences, Department of Chemistry
Swedish University of Agricultural Sciences, Department of Food Science
Bondia-Pons, Isabel
Hanhineva, Kati
University of Eastern Finland
Juntunen, Katri
City Kuopio, Health Care Service
Antl, Nadja
Swedish University of Agricultural Sciences, Department of Chemistry
Antl, Nadja
Swedish University of Agricultural Sciences, Department of Food Science
Poutanen, Kaisa
Mykkänen, Hannu
University of Eastern Finland

UKÄ Subject classification

Nutrition and Dietetics

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