Abstract

In this experimental study a microinjection technique for the administration of lipid-soluble xenobiotics in rainbow trout embryos was applied. One week prior to hatching, rainbow trout embryos were injected in the yolk-sac with commercial blends of polychlorinated biphenyls (PCBs), polychlorinated naphthalenes (PCNs), polychlorinated paraffins (PCPs) and polybrominated diphenyl ethers (PBDEs). Six weeks later, the morphology of the liver was examined, and the hepatic cytochrome P450-dependent ethoxyresorufin-O-deethylase (EROD) activity was determined in the swim-up fry. A dose-dependent induction of the EROD activity was found for the PCBs and the PCNs, indicating that application of the microinjection technique in fish embryo bioassays, combined with studies of liver morphology and biochemistry, facilitate detection of biological responses to certain lipid-soluble substances.

Keywords

MICROINJECTION; RAINBOW TROUT EMBRYO; ORGANIC POLLUTANT; EROD INDUCTION; LIVER MORPHOLOGY

Published in

Aquatic Toxicology
1993, volume: 26, number: 3-4, pages: 307-316
Publisher: ELSEVIER SCIENCE BV

Authors' information