Abstract

Amplified Fragment Length Polymorphism (AFLP) was used to study genetic relationship among leaf rust fungi (Melampsora spp.) occurring on Salix species in short-rotation coppice. Special interest was paid to a newly identified rust fungus found on S. viminalis in southern Sweden, morphologically similar to M. larici-epitea but with a distinct DNA profile. Genetic distances among 40 Melampsora isolates collected from S. viminalis, S. dasyclados, S. viminalis x dasyclados, S. daphnoides, S. acutifolia and M. larici-populina were calculated based on 101 AFLP markers. Neighbour-joining analysis revealed the presence of six clusters, which corresponded exactly to predefined groups, namely three formae speciales of M. larici-epitea, a stem-infecting form of Melampsora on S. viminalis, the newly identified Melampsora on S. viminalis and a group consisting of two isolates from the poplar rust M. larici-populina. All six clusters were well supported by bootstrap analysis (84 to 100% support). The newly identified Melampsora on S. viminalis was indicated to be genetically separated from M. larici-epitea as well as from the stem-infecting form of Melampsora.

Published in

Forest Pathology
2002, Volume: 32, number: 6, pages: 379-386
Publisher: BLACKWELL VERLAG GMBH

SLU Authors

• Samils, Berit

  • Sveriges lantbruksuniversitet, Swedish University of Agricultural Sciences
    

• Lagercrantz, Ulf

  • Sveriges lantbruksuniversitet, Swedish University of Agricultural Sciences
    

• Gullberg, Urban

  • Sveriges lantbruksuniversitet, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Agricultural Science
Forest Science
Genetics


Publication identifier

DOI: https://doi.org/10.1046/j.1439-0329.2002.00298.x

Permanent link to this page (URI)

https://res.slu.se/id/publ/43538