Abstract

Amplified fragment length polymorphism (AFLP) was used to study the genetic structure of populations of the willow leaf rust, Melampsora epitea, in Swedish willow plantations. In total, 197 isolates collected from Salix viminalis clones in three locations in Sweden were analysed. AFLP profiles based on 83 markers were used to compute genetic distances between pairs of individuals. High levels of gene and genotypic diversity were detected in all populations, with 96% of the AFLP loci being polymorphic and with normalized Shannon's diversity indices ranging from 0.977 to 1.0. Analysis of molecular variance (AMOVA) showed small significant differences among locations, although most of the molecular variability was found within locations (97.5%). Five isolates from one willow clone in one location differed markedly from the common pattern. When these five exceptional isolates were excluded, no significant differences among willow clones were found with AMOVA. Sexual reproduction and spore migration appear to be important factors for the population genetic structure of this pathogen.

Keywords

AFLP; AMOVA; diversity; F-statistics; leaf rust; Shannon index

Published in

European Journal of Plant Pathology
2001, Volume: 107, number: 4, pages: 399-409
Publisher: KLUWER ACADEMIC PUBL

SLU Authors

• Samils, Berit

  • Sveriges lantbruksuniversitet, Swedish University of Agricultural Sciences
    

• Lagercrantz, Ulf

  • Sveriges lantbruksuniversitet, Swedish University of Agricultural Sciences
    

• Gullberg, Urban

  • Sveriges lantbruksuniversitet, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Genetics
Forest Science
Agricultural Science


Publication Identifiers

DOI: https://doi.org/10.1023/A:1011270315251

Permanent link to this page (URI)

https://res.slu.se/id/publ/43539