Early Cone Setting in Picea abies acrocona Is Associated with Increased Transcriptional Activity of a MADS Box Transcription Factor

Abstract

Conifers normally go through a long juvenile period, for Norway spruce (Picea abies) around 20 to 25 years, before developing male and female cones. We have grown plants from inbred crosses of a naturally occurring spruce mutant (acrocona). One-fourth of the segregating acrocona plants initiate cones already in their second growth cycle, suggesting control by a single locus. The early cone-setting properties of the acrocona mutant were utilized to identify candidate genes involved in vegetative-to-reproductive phase change in Norway spruce. Poly(A(+)) RNA samples from apical and basal shoots of cone-setting and non-cone-setting plants were subjected to high-throughput sequencing (RNA-seq). We assembled and investigated 33,383 expressed putative protein-coding acrocona transcripts. Eight transcripts were differentially expressed between selected sample pairs. One of these (Acr42124_1) was significantly up-regulated in apical shoot samples from cone-setting acrocona plants, and the encoded protein belongs to the MADS box gene family of transcription factors. Using quantitative real-time polymerase chain reaction with independently derived plant material, we confirmed that the MADS box gene is up-regulated in both needles and buds of cone-inducing shoots when reproductive identity is determined. Our results constitute important steps for the development of a rapid cycling model system that can be used to study gene function in conifers. In addition, our data suggest the involvement of a MADS box transcription factor in the vegetative-to-reproductive phase change in Norway spruce.

Published in

Plant Physiology
2013, volume: 161, number: 2, pages: 813-823
Publisher: AMER SOC PLANT BIOLOGISTS

SLU Authors

• Uddenberg, Daniel

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Clapham, David

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Von Arnold, Sara

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

• Sundström, Jens

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Developmental Biology
Plant Biotechnology

Publication identifier

• DOI: https://doi.org/10.1104/pp.112.207746

Permanent link to this page (URI)

https://res.slu.se/id/publ/43647