- Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences
Kumaresan, A; Johannisson, Anders; Humblot, Patrice; Bergqvist, Ann-Sofie
Following insemination, spermatozoa are retained in the utero-tubal junction and isthmic region of the oviduct, where essential steps of capacitation are coordinated. Although a majority of the spermatozoa is exposed to similar conditions in the oviduct, the speed of the response varies depending on the individual male and the state of the spermatozoa. The present study evaluated individual boar variations in terms of the ability of spermatozoa to undergo tyrosine phosphorylation in response to isthmic oviductal fluid (ODF). Cryopreserved spermatozoa from four boars were incubated with pre- and post-ovulatory ODF for 6?hr. Sperm kinematics, global protein tyrosine phosphorylation, and dynamics of different phosphorylation patterns were analyzed at hourly interval. The percentage of phosphorylated spermatozoa in the pre-ovulatory ODF-treated group was significantly (P?
Molecular Reproduction and Development
2012, Volume: 79, number: 8, pages: 525-540