Seventy-five EST-linked Atlantic salmon (Salmo salar L.) microsatellite markers and their cross-amplification in five salmonid species

Vasemagi A, Nilsson J, Primmer CR

doi:10.1111/j.1471-8286.2005.00902.x

Research article - Peer-reviewed, 2005

Seventy-five EST-linked Atlantic salmon (Salmo salar L.) microsatellite markers and their cross-amplification in five salmonid species

Vasemagi A, Nilsson J, Primmer CR

Abstract

An Atlantic salmon (Salmo salar L.) expressed sequence tag (EST) database consisting of 58 146 ESTs was screened for microsatellite sequences. Subsequent development of 75 polymorphic EST-associated microsatellite markers in this species is described together with cross-species amplification results of 133 gene-associated tandem repeat markers in five salmonid species (Salmo trutta, Oncorhynchus mykiss, Salvelinus aplinus, Thymallus thymallus, Coregonus lavaretus). The number of alleles among EST-linked microsatellites in Atlantic salmon ranged from two to 41 with an average of 12 alleles per locus. Cross-species amplification resulted in detection of a total of ill polymorphic locus-species combinations (12-32 loci per species)

Published in

Molecular Ecology Notes
2005, volume: 5, number: 2, pages: 282-288
Publisher: BLACKWELL PUBLISHING

Authors' information

Vasemägi, Anti

Swedish University of Agricultural Sciences, Department of Aquaculture

Nilsson, Jan

Swedish University of Agricultural Sciences, Department of Aquaculture

Primmer, Craig R

UKÄ Subject classification

Fish and Aquacultural Science

Publication Identifiers

DOI: https://doi.org/10.1111/j.1471-8286.2005.00902.x

URI (permanent link to this page)

https://res.slu.se/id/publ/4463