A new approach for cytokinin isolation from Arabidopsis tissues using miniaturized purification: pipette tip solid-phase extraction

Svačinová, Jana; Novak, Ondrej; Plačková, Lenka; Lenobel, René; Holík, Josef; Strnad, Miroslav; Doležal, Karel

doi:10.1186/1746-4811-8-17

Research article2012Peer reviewedOpen access

A new approach for cytokinin isolation from Arabidopsis tissues using miniaturized purification: pipette tip solid-phase extraction

Svačinová, Jana; Novak, Ondrej; Plačková, Lenka; Lenobel, René; Holík, Josef; Strnad, Miroslav; Doležal, Karel

Abstract

Conclusions: The combination of microextraction with one-step high-throughput purification provides fast, effective and cheap sample preparation prior to qualitative and quantitative measurements. Our procedure can be used after modification also for other phytohormones, depending on selectivity, affinity and capacity of the selected sorbents.

Keywords

Cytokinins; Arabidopsis thaliana; Pipette tip solid-phase extraction (PT-SPE); StageTip; Ultra-high; performance liquid chromatography (UHPLC); Tandem mass spectrometry (MS/MS)

Published in

Plant Methods
2012, Volume: 8
Publisher: BIOMED CENTRAL LTD

SLU Authors

Novak, Ondrej
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences

UKÄ Subject classification

Botany

Publication identifier

DOI: https://doi.org/10.1186/1746-4811-8-17

Permanent link to this page (URI)

https://res.slu.se/id/publ/44832