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Research article - Peer-reviewed, 2012

Redox-mediated Mechanisms Regulate DNA Binding Activity of the G-group of Basic Region Leucine Zipper (bZIP) Transcription Factors in Arabidopsis

Shaikhali, Jehad; Norén, Louise; Barajas-López, Juan de Dios; Srivastava, Vaibhav; König, Janine; Sauer, Uwe H.; Wingsle, Gunnar; Dietz, Karl-Josef; Strand, Åsa


Plant genes that contain the G-box in their promoters are responsive to a variety of environmental stimuli. Bioinformatics analysis of transcriptome data revealed that the G-box element is significantly enriched in promoters of high light-responsive genes. From nuclear extracts of high light-treated Arabidopsis plants, we identified the AtbZIP16 transcription factor as a component binding to the G-box-containing promoter fragment of light-harvesting chlorophyll a/b-binding protein2.4 (LHCB2.4). AtbZIP16 belongs to the G-group of Arabidopsis basic region leucine zipper (bZIP) type transcription factors. Although AtbZIP16 and its close homologues AtbZIP68 and AtGBF1 bind the G-box, they do not bind the mutated half-sites of the G-box palindrome. In addition, AtbZIP16 interacts with AtbZIP68 and AtGBF1 in the yeast two-hybrid system. A conserved Cys residue was shown to be necessary for redox regulation and enhancement of DNA binding activity in all three proteins. Furthermore, transgenic Arabidopsis lines overexpressing the wild type version of bZIP16 and T-DNA insertion mutants for bZIP68 and GBF1 demonstrated impaired regulation of LHCB2.4 expression. Finally, overexpression lines for the mutated Cys variant of bZIP16 provided support for the biological significance of Cys330 in redox regulation of gene expression. Thus, our results suggest that environmentally induced changes in the redox state regulate the activity of members of the G-group of bZIP transcription factors.

Published in

Journal of Biological Chemistry
2012, volume: 287, number: 33, pages: 27510-27525

Authors' information

Shaikhali, Jehad
Norén, Louise
Umeå University
Barajas-López, Juan de Dios
Umeå University
Srivastava, Vaibhav
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
König, Janine
Bielefeld University
Sauer, Uwe H.
Umeå University
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Dietz, Karl-Josef
Bielefeld University
Strand, Åsa
Umeå University

UKÄ Subject classification

Biochemistry and Molecular Biology

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