Research article - Peer-reviewed, 2012
Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species
Schwochow, Doreen; Serieys, Laurel E.K.; Wayne, Robert K.; Thalmann, OlafAbstract
Conclusion: By carefully choosing the appropriate RNA extraction method, whole blood can become a valuable source for high-throughput applications like expression arrays or transcriptome sequencing from natural populations. Additionally, candidate genes showing signs of selection could subsequently be genotyped in large population samples using whole blood as a source for RNA without harming individuals from rare or endangered species.Keywords
Whole blood; RNA preservation; RNA extraction; DNA contamination; Transcriptome sequencing; Globin transcriptsPublished in
BMC Biotechnology2012, volume: 12, pages: 1-11
Publisher: BIOMED CENTRAL LTD
Authors' information
Schwochow, Doreen
Swedish University of Agricultural Sciences, Department of Animal Breeding and Genetics
Serieys, Laurel E.K.
University of California Los Angeles (UCLA)
Wayne, Robert K.
Thalmann, Olaf
University of California Los Angeles (UCLA)
UKÄ Subject classification
Microbiology
Other Environmental Biotechnology
Publication Identifiers
DOI: https://doi.org/10.1186/1472-6750-12-33
URI (permanent link to this page)
https://res.slu.se/id/publ/45166