Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species

Schwochow, Doreen; Serieys, Laurel E.K.; Wayne, Robert K.; Thalmann, Olaf

doi:10.1186/1472-6750-12-33

Research article - Peer-reviewed, 2012

Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species

Schwochow, Doreen; Serieys, Laurel E.K.; Wayne, Robert K.; Thalmann, Olaf

Abstract

Conclusion: By carefully choosing the appropriate RNA extraction method, whole blood can become a valuable source for high-throughput applications like expression arrays or transcriptome sequencing from natural populations. Additionally, candidate genes showing signs of selection could subsequently be genotyped in large population samples using whole blood as a source for RNA without harming individuals from rare or endangered species.

Keywords

Whole blood; RNA preservation; RNA extraction; DNA contamination; Transcriptome sequencing; Globin transcripts

Published in

BMC Biotechnology
2012, volume: 12, pages: 1-11
Publisher: BIOMED CENTRAL LTD

Authors' information

Schwochow, Doreen

Swedish University of Agricultural Sciences, Department of Animal Breeding and Genetics

Serieys, Laurel E.K.

University of California Los Angeles (UCLA)

Wayne, Robert K.

Thalmann, Olaf

University of California Los Angeles (UCLA)

UKÄ Subject classification

Microbiology
Other Environmental Biotechnology

Publication Identifiers

DOI: https://doi.org/10.1186/1472-6750-12-33

URI (permanent link to this page)

https://res.slu.se/id/publ/45166