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Research article2012Peer reviewed

Species specific primers for identification of Alternaria solani, in combination with analysis of the F129L substitution associates with loss of sensitivity toward strobilurins

Edin, Eva


A species specific forward primer was designed in order to identify the phytopathogenic fungus Alternaria solani, which causes early blight on potato. The reverse primer came from another survey of the American population of A. solani. The combination of these two primers can be used both as molecular identification of A. solani as well as sending the PCR product for sequencing in search for the F129L substitution associated with loss of sensitivity toward fungicides based on strobilurins. The diagnostic method has been successfully used when identifying the causal agent of lesions on potato leaflets during large field assays. (C) 2012 Elsevier Ltd. All rights reserved.


Molecular diagnostics; PCR; Species-specific primers; Fungicide resistance; Alternaria solani

Published in

Crop Protection
2012, Volume: 38, pages: 72-73

    Associated SLU-program

    AMR: Fungus

    UKÄ Subject classification

    Agricultural Science

    Publication identifier


    Permanent link to this page (URI)