A fibrinogen-binding protein of Staphylococcus lugdunensis

Abstract

A gene called fbl, encoding a Staphylococcus lugdunensis fibrinogen-binding protein, was identified by phage display. The encoded protein, Fbl, is a member of the Sdr-family, a group of staphylococcal cell surface proteins containing a characteristic serine-aspartate repeat region. The fibrinogen-binding domain was mapped to 313 amino acids, and shows, 62% identity to the corresponding region in clumping factor (ClfA) from Staphylococcus aureus. Anti-serum against ClfA cross-reacted with Fbl, and blocked S. lugdunensis adherence to fibrinogen. Twelve clinical isolates of S. lugdunensis analysed by Southern blot all had an fbl-like gene. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved

Keywords

Staphylococcus lugdunensis; fibrinogen-binding; Sdr-family; LPXTG; clumping facto; phage dispaly

Published in

FEMS Microbiology Letters
2004, Volume: 241, number: 1, pages: 87-93 Publisher: ELSEVIER SCIENCE BV

SLU Authors

• Nilsson, Martina

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Bjerketorp, Joakim

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Frykberg, Lars

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Guss, Bengt

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

Publication identifier

DOI: https://doi.org/10.1016/j.femsle.2004.10.008

Permanent link to this page (URI)

https://res.slu.se/id/publ/4554