Research article - Peer-reviewed, 2012
Molecular Characterization of Feline Infectious Peritonitis Virus Strain DF-2 and Studies of the Role of ORF3abc in Viral Cell Tropism
Balint, Adam; Farsang, Attila; Zadori, Zoltan; Hornyák, Ákos; Dencső, László; Almazán, Fernando; Enjuanes, Luis; Belák, SándorAbstract
The full-length genome of the highly lethal feline infectious peritonitis virus (FIPV) strain DF-2 was sequenced and cloned into a bacterial artificial chromosome (BAC) to study the role of ORF3abc in the FIPV-feline enteric coronavirus (FECV) transition. The reverse genetic system allowed the replacement of the truncated ORF3abc of the original FIPV DF-2 genome with the intact ORF3abc of the canine coronavirus (CCoV) reference strain Elmo/02. The in vitro replication kinetics of these two viruses was studied in CrFK and FCWF-4 cell lines, as well as in feline peripheral blood monocytes. Both viruses showed similar replication kinetics in established cell lines. However, the strain with a full-length ORF3 showed markedly lower replication of more than 2 log(10) titers in feline peripheral blood monocytes. Our results suggest that the truncated ORF3abc plays an important role in the efficient macrophage/monocyte tropism of type II FIPV.Published in
Journal of Virology2012, volume: 86, number: 11, pages: 6258-6267
Publisher: AMER SOC MICROBIOLOGY
Authors' information
Balint, Adam
Veterinary Diagnostic Directorate
Balint, Adam
Swedish University of Agricultural Sciences, Department of Biomedical Science and Veterinary Public Health
Farsang, Attila
Directorate of Veterinary Medicinal Products
Zadori, Zoltan
Hungarian Academy of Sciences
Zadori, Zoltan
Swedish University of Agricultural Sciences, Department of Biomedical Science and Veterinary Public Health
Hornyák, Ákos
Dencső, László
Central Agricultural Office
Almazán, Fernando
Centro Nacional de Biotecnología (CNB-CSIC)
Enjuanes, Luis
Centro Nacional de Biotecnología (CNB-CSIC)
Belák, Sándor (Belak, Sandor)
Swedish University of Agricultural Sciences, Department of Biomedical Science and Veterinary Public Health
Belák, Sándor (Belak, Sandor)
National Veterinary Institute (SVA)
UKÄ Subject classification
Cell and Molecular Biology
Publication Identifiers
DOI: https://doi.org/10.1128/JVI.00189-12
URI (permanent link to this page)
https://res.slu.se/id/publ/45565