Abstract

Immunoreaction for TNF was found in all samples used. In the dog testis, TNF immunoexpression was limited to the seminiferous tubules, where late round spermatids (SPD) showed weak intensity of immunostaining, while elongating and elongated SPD evidenced moderate and the residual bodies a strong intensity. In the epididymis, a gradual progressive increase of TNF immunolabelling was found throughout the epididymal regions, ranging from a weak intensity at the caput epididymis to a moderate intensity at the cauda. TNF immunolabelling was found in mature SPZ during the epididymal transit and also in freshly ejaculated SPZ, which showed a strong midpiece immunolabelling. Data presented here provide important information on expression of TNF in spermatozoa, which is acquired by the SPZ during their formation at the testis. It further provides the basis for subsequent studies on the physiological importance of cytokines in sperm function. (c) 2012 Elsevier Inc. All rights reserved.

Keywords

Tumor necrosis factor-alpha; Spermatogenesis; Testis; Spermatozoa; Dog

Published in

Theriogenology
2012, Volume: 77, number: 8, pages: 1540-1548
Publisher: ELSEVIER SCIENCE INC

SLU Authors

• Ström Holst, Bodil

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Pathobiology


Publication identifier

DOI: https://doi.org/10.1016/j.theriogenology.2011.11.021

Permanent link to this page (URI)

https://res.slu.se/id/publ/45618