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Research article - Peer-reviewed, 2012

Hydroperoxide and peroxynitrite reductase activity of poplar thioredoxin-dependent glutathione peroxidase 5: kinetics, catalytic mechanism and oxidative inactivation

Selles, Benjamin; Hugo, Martin; Trujillo, Madia; Srivastava, Vaibhav; Wingsle, Gunnar; Jacquot, Jean-Pierre; Radi, Rafael; Rouhier, Nicolas

Abstract

Gpxs (glutathione peroxidases) constitute a family of peroxidases, including selenocysteine- or cysteine-containing isoforms (SeCys-Gpx or Cys-Gpx), which are regenerated by glutathione or Trxs (thioredoxins) respectively. In the present paper we show new data concerning the substrates of poplar Gpx5 and the residues involved in its catalytic mechanism. The present study establishes the capacity of this Cys-Gpx to reduce peroxynitrite with a catalytic efficiency of 10(6) M-1.s(-1). In PtGpx5 (poplar Gpx5; Pt is Populus trichocarpa), Glu(79), which replaces the glutamine residue usually found in the Gpx catalytic tetrad, is likely to be involved in substrate selectivity. Although the redox midpoint potential of the Cys(44)-Cys(92) disulfide bond and the pK(a) of Cys(44) are not modified in the E79Q variant, it exhibited significantly improved kinetic parameters (K-peroxide and k(cat)) with tert-butyl hydroperoxide. The characterization of the monomeric Y151R variant demonstrated that PtGpx5 is not an obligate homodimer. Also, we show that the conserved Phe(90) is important for Trx recognition and that Trx-mediated recycling of PtGpx5 occurs via the formation of a transient disulfide bond between the Trx catalytic cysteine residue and the Gpx5 resolving cysteine residue. Finally, we demonstrate that the conformational changes observed during the transition from the reduced to the oxidized form of PtGpx5 are primarily determined by the oxidation of the peroxidatic cysteine into sulfenic acid. Also, MS analysis of in-vitro-oxidized PtGpx5 demonstrated that the peroxidatic cysteine residue can be over-oxidized into sulfinic or sulfonic acids. This suggests that some isoforms could have dual functions potentially acting as hydrogen-peroxide- and peroxynitrite-scavenging systems and/or as mediators of peroxide signalling as proposed for 2-Cys peroxiredoxins.

Keywords

glutathione peroxidase; over-oxidation; peroxynitrite; redox property; site-directed mutagenesis; thiol peroxidase

Published in

Biochemical Journal
2012, Volume: 442, number: 2, pages: 369-380
Publisher: PORTLAND PRESS LTD