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Research article - Peer-reviewed, 2012

How MIKC* MADS-Box Genes Originated and Evidence for Their Conserved Function Throughout the Evolution of Vascular Plant Gametophytes

Kwantes, Michiel; Liebsch, Daniela; Verelst, Wim

Abstract

Land plants have a remarkable life cycle that alternates between a diploid sporophytic and a haploid gametophytic generation, both of which are multicellular and changed drastically during evolution. Classical MIKC MADS-domain (MIKC(C)) transcription factors are famous for their role in sporophytic development and are considered crucial for its evolution. About the regulation of gametophyte development, in contrast, little is known. Recent evidence indicated that the closely related MIKC* MADS-domain proteins are important for the functioning of the Arabidopsis thaliana male gametophyte (pollen). Furthermore, also in bryophytes, several MIKC* genes are expressed in the haploid generation. Therefore, that MIKC* genes have a similar role in the evolution of the gametophytic phase as MIKC(C) genes have in the sporophyte is a tempting hypothesis. To get a comprehensive view of the involvement of MIKC* genes in gametophyte evolution, we isolated them from a broad variety of vascular plants, including the lycophyte Selaginella moellendorffii, the fern Ceratopteris richardii, and representatives of several flowering plant lineages. Phylogenetic analysis revealed an extraordinary conservation not found in MIKC(C) genes. Moreover, expression and interaction studies suggest that a conserved and characteristic network operates in the gametophytes of all tested model organisms. Additionally, we found that MIKC* genes probably evolved from an ancestral MIKC(C)-like gene by a duplication in the Keratin-like region. We propose that this event facilitated the independent evolution of MIKC* and MIKC(C) protein networks and argue that whereas MIKC(C) genes diversified and attained new functions, MIKC* genes retained a conserved role in the gametophyte during land plant evolution.

Keywords

MIKC*; gametophyte; K-domain; evolution; gene duplication; MADS

Published in

Molecular Biology and Evolution
2012, Volume: 29, number: 1, pages: 293-302
Publisher: OXFORD UNIV PRESS

    UKÄ Subject classification

    Biochemistry and Molecular Biology
    Genetics
    Evolutionary Biology

    Publication identifier

    DOI: https://doi.org/10.1093/molbev/msr200

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/45909