Morrell, Jane
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
Abstract
Ejaculates from 7 stallions were split and simultaneously frozen in three different extenders, INRA 96 egg yolk glycerol, Ghent and the newly developed extender Caceres. After thawing, samples were evaluated for motility (CASA system) sperm membrane integrity and early membrane changes (YoPro-1/Eth staining), acrosome integrity (FICT-PNA), and mitochondrial membrane potential (JC-1) (flow cytometry). Samples frozen in Caceres extender consistently showed the best results in post-thaw motility (increases ranging from 11 to 17%, p < 0.05) and velocity (p < 0.05), membrane integrity (increases ranging from 11 to 14%, p < 0.05) and mitochondrial membrane potential (p < 0.05). It is concluded that this new extender should be included in a freezeability test to determine the best extender for each individual. (C) 2011 Elsevier B.V. All rights reserved.
Keywords
Stallion; Frozen sperm; Flow cytometry; CASA
Published in
Animal Reproduction Science
2011, volume: 127, number: 1-2, pages: 78-83
Publisher: ELSEVIER SCIENCE BV
SLU Authors
UKÄ Subject classification
Veterinary Science
Animal and Dairy Science
Publication identifier
- DOI: https://doi.org/10.1016/j.anireprosci.2011.07.009
Permanent link to this page (URI)
https://res.slu.se/id/publ/46251