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Research article - Peer-reviewed, 2011

Survey of bromodeoxyuridine uptake among environmental bacteria and variation in uptake rates in a taxonomically diverse set of bacterial isolates

Hellman, Maria; Berg, Jeanette; Brandt, Kristian K.; Hallin, Sara

Abstract

Incorporation of 5-Bromo-2'-Deoxyuridine (BrdU) into DNA can be used to target replicating bacteria in the environment, but differential uptake capacity is a potential bias. Among 23 bacterial isolates commonly found in soils, most took up BrdU, but at up to 10-fold different cell-specific rates. Combined with results from an in silico analysis of 1000 BrdU-labeled 16S rRNA gene sequences, our results demonstrate a BrdU uptake bias with no apparent relationship between taxa affiliation and ability to incorporate BrdU. (C) 2011 Elsevier B.V. All rights reserved.

Keywords

Active bacteria; BrdU incorporation; Replicating bacteria; Soil bacteria; Thymidine analog

Published in

Journal of Microbiological Methods
2011, Volume: 86, number: 3, pages: 376-378
Publisher: ELSEVIER SCIENCE BV

      SLU Authors

    • UKÄ Subject classification

      Agricultural Science
      Fish and Aquacultural Science
      Environmental Sciences related to Agriculture and Land-use

      Publication identifier

      DOI: https://doi.org/10.1016/j.mimet.2011.05.020

      Permanent link to this page (URI)

      https://res.slu.se/id/publ/46481